Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 DH5αpir116 variant (LMBP 7961)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | DH5αpir116 variant |
| Accession number: | LMBP 7961 |
| Depositor: | Prof. Dr M. Herrington1 1 Biology Dept, Concordia University, Montreal, Canada It was obtained from Dr G. Philips2 2 College of Veterinary Medicine, Dept of Microbiology, Iowa State University, Ames, Iowa, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Miller |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 Φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir116 |
| Phenotype: | TetS NalS |
| Properties: | This is a host strain for R6Kγori plasmids. In pir116 strains, R6K-containing plasmids replicate like high-copy pUC plasmids. |
| Further information: | Personal communications of Prof. Dr M. Herrington: The E. coli K12 DH5αpir116 variant appears to have lost the Tn10 and is consequently tetracycline sensitive. BCCM/GeneCorner could not experimentally confirm the nalidixic acid resistance, conferred by gyrA96, nor the tetracycline resistance, conferred by Tn10. |
| Original reference: | Platt et al., Plasmid 43 (2000), 12-23 [PMID: 10610816] |
| Related reference: | Sitaras et al., Plasmid 65 (2011), 232-238 [PMID: 21376749] [DOI: 10.1016/j.plasmid.2011.02.005] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 DH5αpir116 variant (LMBP 7961) is available at BCCM/GeneCorner. It was deposited by Prof. Dr M. Herrington and was published in Platt et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.